Device

Part:BBa_K1357003:Design

Designed by: Matthew Tucker   Group: iGEM14_Virginia   (2014-10-06)


IPTG Inducible NhaR Expression Construct


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 256


Design Notes

The expression of NhaR was preliminarily put under the influence of an IPTG inducible promoter. After observing the results, a strong constitutive promoter was opted for.


Source

The NhaR gene in this part comes for E. coli the K-12 genome. The remainder of the parts were obtained from the parts distribution kits.

References